By Helmut Breuninger, Patrick Adam
There hasn’t been a publication pertaining to "Microscopically managed surgical procedure" released and it is important to put up a e-book that information the entire diverse phrases and technique utilized in microscopically managed surgical procedure. The aim is to create a pragmatic, concise and easy rationalization of 3D-histology with workflows and distinctive illustrative fabric for dermatologists. it truly is consequently designed to be a goal-oriented guide instead of an exhaustive reference paintings. it's going to give you the crucial details for all operating with sufferers present process this crew of treatments.
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Extra info for 3D Histology Evaluation of Dermatologic Surgery
2c). Deeper sections are necessary until the margin color is present in the whole plane of the embedded specimen (Fig. 2d, see also Fig. 11). Therefore, the staining helps to determine the depth of the sections until the entire margin is covered. Dividing an Excised Specimen into Two Margin Strips and Base (Vertical Excision, Paraffin Embedding) The differences between oblique and vertical excision have been discussed previously and it remains to the individual surgeon which excision technique is preferred (see Chap.
On the right, the vertical excision procedure is shown (Fig. 8b). Excising the Tumor 23 12 o’clock position is turned! 9 Spindle-shaped vertical excision and flattening of the margins. 12 o’clock marker at the upper point These basic principles, oblique and vertical excision, tumor and safety margin excision in multiple excisions, or one with following preparation of the central tumor, are the core of any surgical procedure for skin tumors. In the following, two other variants of excision methods using vertical excisions are described.
Then the slides are ready for H&E staining and the entire margins of the excised tumor can be evaluated in a full histological workup (Fig. 5d). Division and Flattening of a Formalin-Fixed Tumor Specimen As mentioned before, the margins and base of the excised tumor can also be prepared by a histopathological laboratory once the tissue has already been fixed in formalin . 6 Preparation of a formalin-fixed tumor specimen. Flattening of the strips and base is possible if paraffinized tissue is warmed up Much of the procedure follows the previously described preparation of native tissue (see figures in Chap.