By Jianhua Zhao, Haishan Zeng (auth.), Rongguang Liang (eds.)
This e-book offers an creation to layout of biomedical optical imaging applied sciences and their purposes. the most subject matters contain: fluorescence imaging, confocal imaging, micro-endoscope, polarization imaging, hyperspectral imaging, OCT imaging, multimodal imaging and spectroscopic platforms.
Each bankruptcy is written through the realm leaders of the respective fields, and should conceal:
- principles and barriers of optical imaging technology,
- system layout and useful implementation for one or particular purposes, together with layout directions, approach configuration, optical layout, part requisites and choice, procedure optimization and layout examples,
- recent advances and purposes in biomedical researches and scientific imaging.
This e-book serves as a reference for college students and researchers in optics and biomedical engineering.
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Additional resources for Biomedical Optical Imaging Technologies: Design and Applications
2) The measurement time is limited by the clinical procedure. The measurement time allowed for an in vivo application is less than a couple of seconds. (3) Raman signal of lung tissue is very weak. Real-time in vivo Raman imaging of macroscopic tissue area is technologically infeasible. Therefore, Raman spectroscopy itself cannot be used as an efficient method for locating the lesion. We have to rely on autofluorescence or white light imaging to locate the sites of concern and then apply the probe for Raman spectra measurement.
The surrounding 15 fibers (200-m core diameter, NA D 0:27) were used for signal collection. There were two filters between the fiber tip and the ball lens. The central rodlike short-pass filter was used to reject Raman and fluorescence signal from the fiber, and the other tubelike long-pass filter was used to reject the elastically scattered laser light. There was a metal sleeve between the two filters to prevent cross talk. 75 mm in outer diameter. Shim et al.  developed an endoscopic probe for colon cancer diagnosis, similar to the ball-based probe (Fig.
Four hundred and ninety-four Raman spectra at different depths and from normal and tumor-bearing sites of 24 mice were measured. We found different spectral patterns at different depths. A peak at 899 cm 1 (possibly from proline or fatty acids) and one with higher intensity in 32 J. Zhao et al. Fig. 16 Depth-resolved confocal Raman spectra of (a) normal mouse skin and (b) tumor-bearing skin in vivo (average of 24 mice) (Adapted from Wang et al. , with permission) 1 Advanced Spectroscopy Technique for Biomedicine 33 the 1;325–1;330 cm 1 range (assigned to nucleic acids) were correlated with the presence of tumors, which can potentially be used as biomarkers for skin cancer detection.