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CI 975b) investigated the influence of aseries of proteins on the thermotropic properties of synthetic phospholipids. The proteins can be divided into three classes according to the effects they exert on the melting profiles of the lipids: 22 Biomembrane Structure and Function 1. Those that cause a decrease in MI and Tm (cytochrome c and A1-myelin basic protein). The primary interaction is an electrostatic one, followed by limited penetration of the protein into the bilayer. 2. Those that have almost no influence on Tm, but cause a decrease in MI (proteolipid apoprotein from myelin, the peptide gramicidin A).