By Rosalyn D. Blumenthal
A state-of-the artwork selection of simply reproducible laboratory equipment for assessing chemosensitivity in vitro and in vivo, and for assessing the parameters that modulate chemosensitivity in person tumors. Chemosensitivity, quantity 1: In Vitro Assays offers a panel of sixteen in vitro measures of chemosensitivity in adherent and non-adherent cells for unmarried brokers and combos of brokers. as well as immunohistochemical and imaging ways, those assays contain clonogenic, colorimetric, fluorometric, and physiological assays. Highlights comprise snapshot research to evaluate drug sensitivity, excessive throughput methods utilizing eco-friendly fluorescent protein, DIMSCAN (a microcomputer fluorescence-based assay), and the ChemoFx assay utilized in biotechnology. A spouse quantity, quantity 2: In Vivo versions, Imaging, and Molecular Regulators, presents protocols for classifying tumors into reaction different types and customizing chemotherapy regimens to person sufferers.
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Additional info for Chemosensitivity: Volume I: In Vitro Assays (Methods in Molecular Medicine)
Automated microplate reader (enzyme-linked immunosorbent assay [ELISA] reader). 3. Methods All cell culture work must be performed under sterile working conditions and under standard cell culture conditions (humidified air, 5% CO2, and 37°C) or adapted to requirements of the specific cell lines, if necessary. 1. Seeding of Microtiter Plates for Growth Kinetics For cytotoxicity experiments, it is critical to ensure exponential cell growth for the entire duration of the assay. In particular, this is dependent on the number of cells seeded per well in the microtiter plate at the initiation of the experiment.
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