By Antonia Vlahou, Manousos Makridakis
This moment editionexpands upon the former version with present, special advancements within the box and brings jointly a multi-disciplinary crew of best researchers to supply their most modern protocols for scientific proteomics research. Written within the hugely profitable Methods in Molecular Biology sequence structure, chapters contain introductions to their respective themes, lists of the required fabrics and reagents, step by step, comfortably reproducible laboratory protocols, and pointers on troubleshooting and fending off recognized pitfalls.
Authoritative and useful, Clinical Proteomics: tools and Protocols, moment Edition provides techniques that would function a competent consultant to researchers, together with clinicians, chemists, molecular biologists, bioinformaticians and computational, biologists, and investigators engaged on biomarker development.
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Additional info for Clinical Proteomics: Methods and Protocols
J Proteome Res 12(4): 1700–1709 30. Casado B, Iadarola P, Pannell LK (2008) Preparation of nasal secretions for proteome analysis. Methods Mol Biol 425:77–87 31. Lamy E, Mau M (2012) Saliva proteomics as an emerging, non-invasive tool to study livestock physiology, nutrition and diseases. J Proteomics 75:4251–4258 32. Vitorino R, Guedes S, Manadas B et al (2012) Toward a standardized saliva proteome analysis methodology. J Proteomics 75:5140–5165 33. He H, Sun G, Ping F et al (2011) A new and preliminary three-dimensional perspective: proteomes of optimization between OSCC and OLK.
Serum-supplemented growth medium: DMEM (Dulbecco’s modified Eagle medium), High Glucose, GlutaMAX™, Pyruvate, supplemented with 10 % (v/v) FBS and 1 % (v/v) Penicillin/Streptomycin (P/S) (see Note 1). Store at 4 °C (see Note 2). 2. Phosphate-Buffered Saline: 1× in sterile Ultra Pure Water. 34 Agnieszka Latosinska et al. Fig. 3 Comparative analysis of identified proteins from secretome (classical and non-conventional approach) and total cell extract. (I) Comparison of all identified proteins. Venn diagrams representative of all identified proteins from conditioned medium (a) and ER/Golgi fraction (b).
Bronchoalveolar Lavage. php 60. Pastor MD, Nogal A, Molina-Pinelo S et al (2013) Identification of proteomic signatures associated with lung cancer and COPD. J Proteomics 89:227–237 61. Kosanam H, Sato M, Batruch I et al (2012) Differential proteomic analysis of bronchoalveolar lavage fluid from lung transplant patients with and without chronic graft dysfunction. Clin Biochem 45:223–230 62. Cederfur C, Malmstrom J, Nihlberg K et al (2012) Glycoproteomic identification of galectin-3 and -8 ligands in bronchoalveolar lavage of mild asthmatics and healthy subjects.