By Annette M. Griffin, Hugh G. Griffin
Institute of meals learn, Norwich, U.K. equipment in Molecular Biology sequence, quantity 24. First of a two-part useful reduction to the researcher who makes use of pcs for the purchase, garage, or research of nucleic acid or protein sequences. Plastic comb binding. eleven members, three U.S.
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Extra info for Computer Analysis of Sequence Data Part 1 (Methods in Molecular Biology)
Partial) output from Method 3. 3. Selection of the Enzymes The two recommended replies to the question asked are either “*” for selecting all enzymes, or “**” for selecting all enzymes, including the isoschizomers. ” will explain the available options. 4. Definition of the Translation Scheme In this method, it should be known at what reading frame translation occurs. For clarity, this should be the only reading frame to be displayed. , “B” will give three-letter translation of the second reading frame.
The program will note this inclusion as “comment,” which is shown between the heading and the sequence line. 5. Deletion of a Sequence Fragment Being on the command line, the command S,f delete will delete parts of the sequence being edited, where s and f must be sequence coordinates. Note that the coordinates of sequence fragments included after the deletion will change accordingly. 6. Creation of a New Sequence Fragment Being on the command line, the command S,f write will write a new sequence (sequence name is asked if not supplied after the word “write”) with the symbol number 1 being the symbol at the coordinate S, and the end being the numberJ: 4.
Instead, the GCG programs supply the seqed program, which is a powerful tool to enter, manipulate, and store sequences in From Edlted Methods In Molecular Biology, Vol 24’ Computer Analysis of Sequence Data, Part I by A M. Gnffln and H G Gnffm Copyright 01994 Humana Press Inc , Totowa, NJ 57 58 DGZ the correct sequenceformat. Method 1will describehow to edit a sequence from scratch. Method 2 describestools for manipulating the sequence,as well as how to use existing sequences to generate new constructs.