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By Bernard L. Trumpower

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T. Yasunobu, H. F. Mower, and O. ), pp. 182-227. Ple­ num, New York. Salerno, J. , King, T. , and Ohnishi, T. (1979). J. Biol. Chem. 254, 4828-4935. Albracht, S. P. J. (1980). Biochim. Biophys. Acta 612, 11-28. Zeylemaker, W. , Dervartanian, D. , and Veeger, C. (1965). Biochim. Biophys. Acta 99, 183. King, T. E. (1964). Biochem. Biophys. Res. Commun. 16, 511. Ackrell, B. , Kearney, E. , and Singer, T. P. (1978). In "Biomembranes: Part D: Biological Oxidations, Mitochondrial and Microbial Systems".

35. Palmer, G. (1978). In "Biomembranes: Part D: Biological Oxidations, Mitochondrial and Microbial Systems' 1 (S. Fleischer and L. ), Methods in Enzymology, Vol. 53, pp. 113-121. Academic Press, New York. 36. , and King, T. E. (1977). Biochem. Biophys. Res. Commun. 79, 939946. 37. , and King, T. E. (1977). Biochem. Biophys. Res. Commun. 78, 259265. 38. , and Massey, V. (1971). In "Flavins and Flavoproteins" (H. ), pp. 123-136. Uni v. Park Press, Baltimore, Maryland. 39. King, T. E. (1967). 'Oxidation and Phosphorylation" (R.

Protic solutions were 80% ethanol-water by weight. , and acetic acid from Mallinckrodt Chemical Co. (Paris, Kentucky) as specified in the text. Adjustment of pH was made with potassium hydroxide or hydro­ chloric acid. Electrolysis experiments and cyclic voltammetry were performed with an ςς adder" type operational amplifier potentiostat (28) constructed at the electronics facility of the Chemistry Department, Northwestern Univer­ sity. The potentiostat was used together with a voltage generator which provided a reference voltage of either a constant voltage or a triangular wave form with 1 mV incremental steps.

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