Download Long Non-Coding RNAs: Methods and Protocols by Yi Feng, Lin Zhang PDF

By Yi Feng, Lin Zhang

This quantity assembles a large spectrum of tools utilized in lengthy non-coding RNAs (lncRNA) learn, starting from computational annotation of lncRNA genes to molecular and mobile analyses of the functionality of person lncRNA. Long Non-Coding RNAs: tools and Protocols additionally discusses tools used to check round RNAs and RNA splicing, in addition to influential findings on lncRNA in human illnesses. Written within the hugely winning tools in Molecular Biology sequence layout, chapters contain introductions to their respective issues, lists of the mandatory fabrics and reagents, step by step, effectively reproducible laboratory protocols, and pointers on troubleshooting and keeping off identified pitfalls.
Thorough and state of the art, Long Non-Coding RNAs: equipment and Protocols is a must have for molecular biologists, phone and developmental biologists, experts who behavior disease-oriented learn, and bioinformatics specialists who search a greater knowing on lncRNA expression and serve as through computational research of the large sequencing information which are speedily amassing in fresh years.

Show description

Read or Download Long Non-Coding RNAs: Methods and Protocols PDF

Similar anatomy books

The Limbic Brain

This e-book is a needs to for a person drawn to studying concerning the mind. it truly is filled with references to the literature of unique investigators and never basically explains the functionality of the limbic mind, yet, in essence, information the heritage of principles in regards to the limbic mind, from Broca's anatomic description to Papez's Circuit.

Light microscopic techniques in biology and medicine

As much as approximately twenty-five years in the past, almost the complete box of microscopy will be overseen or even practized by means of any lively learn employee. The quick evolution which microscopy in its broadest feel has given that passed through and which has contributed tremendously to our perception in lots of fields of organic technology and drugs has, although, bring about a innovative specialisation.

Extra resources for Long Non-Coding RNAs: Methods and Protocols

Sample text

Nat Rev Genet 11:559–571 2. Mercer TR, Dinger ME, Mattick JS (2009) Long non-coding RNAs: insights into functions. Nat Rev Genet 10:155–159 3. Hammond SM (2007) MicroRNAs as tumor suppressors. Nat Genet 39:582–583 4. Croce CM (2009) Causes and consequences of microRNA dysregulation in cancer. Nat Rev Genet 10:704–714 5. Esteller M (2011) Non-coding RNAs in human disease. Nat Rev Genet 12:861–874 6. Ponting CP, Oliver PL, Reik W (2009) Evolution and functions of long noncoding RNAs. Cell 136:629–641 7.

To ensure result reproducibility, the cells need to be maintained consistently. 3. The abundances of different target protein and lncRNAs may vary from cell line to cell line; therefore the amount of lysate input needs to be empirically determined for each assay. 0 × 107 cells is a good starting point. In cases more cells are needed, scale up the amount of buffer used to ensure high nuclear lysing efficiency. 4. The amount of antibody used for each experiment needs to be empirically determined.

Hum Mol Genet 17:642–655 16. Petrovics G, Zhang W, Makarem M, Street JP, Connelly R, Sun L et al (2004) Elevated expression of PCGEM1, a prostate-specific gene with cell growth-promoting function, is associated with high-risk prostate cancer patients. Oncogene 23:605–611 17. Wang X, Arai S, Song X, Reichart D, Du K, Pascual G et al (2008) Induced ncRNAs allosterically modify RNA-binding proteins in cis to inhibit transcription. Nature 454:126–130 18. Azzalin CM, Reichenbach P, Khoriauli L, Giulotto E, Lingner J (2007) Telomeric repeat containing RNA and RNA surveillance factors at mammalian chromosome ends.

Download PDF sample

Rated 4.79 of 5 – based on 26 votes