By Yi Feng, Lin Zhang
This quantity assembles a large spectrum of tools utilized in lengthy non-coding RNAs (lncRNA) learn, starting from computational annotation of lncRNA genes to molecular and mobile analyses of the functionality of person lncRNA. Long Non-Coding RNAs: tools and Protocols additionally discusses tools used to check round RNAs and RNA splicing, in addition to influential findings on lncRNA in human illnesses. Written within the hugely winning tools in Molecular Biology sequence layout, chapters contain introductions to their respective issues, lists of the mandatory fabrics and reagents, step by step, effectively reproducible laboratory protocols, and pointers on troubleshooting and keeping off identified pitfalls.
Thorough and state of the art, Long Non-Coding RNAs: equipment and Protocols is a must have for molecular biologists, phone and developmental biologists, experts who behavior disease-oriented learn, and bioinformatics specialists who search a greater knowing on lncRNA expression and serve as through computational research of the large sequencing information which are speedily amassing in fresh years.
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Extra resources for Long Non-Coding RNAs: Methods and Protocols
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To ensure result reproducibility, the cells need to be maintained consistently. 3. The abundances of different target protein and lncRNAs may vary from cell line to cell line; therefore the amount of lysate input needs to be empirically determined for each assay. 0 × 107 cells is a good starting point. In cases more cells are needed, scale up the amount of buffer used to ensure high nuclear lysing efficiency. 4. The amount of antibody used for each experiment needs to be empirically determined.
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