Download Nucleic Acids and Protein Synthesis Part G by Kivie Moldave, Lawrence Grossman PDF

By Kivie Moldave, Lawrence Grossman

The severely acclaimed laboratory average, Methods in Enzymology, is likely one of the such a lot hugely revered guides within the box of biochemistry. for the reason that 1955, every one quantity has been eagerly awaited, usually consulted, and praised by means of researchers and reviewers alike. The sequence comprises a lot fabric nonetheless proper at the present time - really a necessary booklet for researchers in all fields of lifestyles sciences

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Additional info for Nucleic Acids and Protein Synthesis Part G

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3 , and - 1 2 . 3 ppm, which must therefore contain at least 3 protons each. The minimum value for the integrated intensity between - 1 1 and - 1 5 ppm is therefore 26 base pairs. VvV'VVV -15 -14 -15 -12 -II -I0 -9 FIG. 2. The 360 MHz NMR spectrum of the minor isoacceptingEscheriehia coli tRNAMa species (tRNA3Ma). The spectrum was obtained as described in Fig. 0. 1~B. R. Reid, Nature (London) 262, 424 (1976). 14C. R. Jones and D. R. Kearns, Proc. Natl. Acad. Sci. A. 71, 4237 (1974). 15K. L. Wong, D.

100 mM to obtain a spectrum of adequate signal intensity under single-sweep conditions. Such concentrations are greatly in excess of the solubility of biological polymers. The spectrum therefore is swept repetitively for several hundred sweeps (signal averaging) and the data are accumulated in a computer of average transients (CAT). The signal-averaged spectrum can be examined at various time intervals and accumulation terminated on attaining an adequate signal-to-noise (S/N) ratio. A S/N of ca.

Coli t R N A TM (lower) in the presence and in the absence o f cobalt. The spectra before and after addition o f cobaltous ion (stippled) are superimposed for ease o f comparison. The initial spectra were taken in 10 m M N a cacodylate, 100 mM NaCI, and 15 m M MgCI=; the cobalt spectra were taken on the same sample after addition o f COC12 to a final concentration o f 0. l raM. as well as lesser effects elsewhere in the spectrum (the shaded Co 2+ spectra are slightly underscaled because of the small dilution effect of the added CoCI~).

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